Influence of mechanical stress on the proliferation and mineralization of human dental pulp stem cells in vitro
XIAO Min
CHEN Bo
LI Ming-wei
HE Wen-xi
YU Qing
Abstract：AIM:To explore the effects of mechanical stress on the proliferation and differentiation of human dental pulp stem cells(hDPSCs).METHODS:Human DPSCs were cultured and subjected to cyclic mechanical stress at 170 g,210 g and 250 g respectively for 30 min every day.Untreated DPSCs served as controls.After that,cell pro-liferation and ALP activity was examined by CCK-8 and ALP kit respectively.The formation of mineralized nodules was determined using half-quantitative Alizarin Red S assay.Cell ultrastructure was observed by the transmission electron microscope.RESULTS:CCK-8 results demonstrated that cell proliferation was reduced(P<0.05),ALP activity and mineralization capacity of stress-treated DPSCs were descended by the stress treatment.The 250 g group had the most significant inhibition(P<0.05).CONCLUSION:Cyclic mechanical stress can inhibit the proliferation and minerali-zation of hDPSCs in vitro in a stress value dependent manner.
Keywords：human dental pulp stem cellscyclic mechanical stressproliferationmineralization
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 187-192 )