Role of NF-κB in condylar cartilage defects repair by pressure-regulated BMSCs/PRF in rabbits

SHI Lei; LI Yi-jie; ZHAO Ying; ZHAO Yin-hua; ZOU De-hui; ZHANG Min; CHEN Yong-jin

doi:10.15956/j.cnki.chin.j.conserv.dent.2015.03.001

Abstract：Abstract] AIM:To investigate the role of NF-κB signaling pathway in condylar cartilage defect repair by pressure-regulated BMSCs/PRF in rabbits.METHODS:Bone marrow mesenchymal stem cells (BMSCs)were cultured from thirty 3 -4 m old New Zealand rabbits and prepared into cell sheets.Platelet-rich fibrin (PRF)were prepared from the arterial blood of the same rabbit.BMSCs/PRF construct was made.Defects of 3 mm in diameter were made on bilat-eral condylar cartilage of the rabbits.The animals were divided into 5 groups (n =6)and the defects were repaired with BMSCs/PRF construct,pressure-regulated BMSCs/PRF,PDTC-pretreated BMSCs/PRF and PDTC-pretreated pressure-regulated BMSCs/PRF,respectively.Animals without treatment served as the controls.2,4 and 8 weeks after opera-tion,the animals were sacrificed and the condylar cartilage were taken.HE staining was used to observe the defect re-pair.I-κB,p-65,aggrecan and Sox-9 mRNA expression was determined by real-time PCR.RESULTS:Pressure-reg-ulated BMSCs/PRF group showed significantly better tissue healing and higher expression of I-κB,p-65,Aggrecan and Sox-9 than the other groups (P <0.05).PDTC treatment significantly reduced I-κB,p-65,Aggrecan and Sox-9 ex-pression (P <0.05).Furthermore,PDTC-treated groups showed reduced defect repair effect and the expvession of I-κB,p-65,aggrecan and Sox-9.CONCLUSION:Pressure-regulated BMSCs/PRF may promote condylar cartilage defect repair.NF-κB signaling pathway is involved in the repair of condylar cartilage defects.