Expression of Periositn mRNA and protein in cultured human periodontal ligament cells after cyclic stretch
LAI Ling-zhi
WU Bu-ling
XU Hui-yong
CHEN Qiu-yue
LIU Qing-na
Abstract：AIM:To observe the effects of cyclic stretch on the expression of periositn(PN)in human perio-dontal ligament cells(hPDLCs).METHODS:Primary hPDLCs were cultured by tissue block enzymolytic method. Cells were subjected to cyclic stretch by Flexcell FX-5000T system at 0.5 Hz of 10% elongation for 6,12,24 and 48 hours respectively.The mRNA and protein expression of PN were measured by qRT-PCR and western blot respective-ly.RESULTS:PN mRNA and protein were positively expressed in control hPDLCs.PN mRNA and protein were de-creased after 6-hour cyclic stretch,then increased after 12-hour cyclic stretch and reached to the highest level after 24-hour treatment(P <0.05).After 48 hour treatment,PN expression began to decline,consistent with the expres-sion level of the control cells.CONCLUSION:Cyclic stretch may change the expression level of PN in hPDLCs in a time-dependent manner.PN may plays an important role in cell signal transduction of mechanical strain.
Keywords：cyclic stretchhuman periodontal ligament cells(hPDLCs)periositn(PN)periodontal tissue re-modeling
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 79-83 )