Immunolocalization of cyclophilin-A in human apical periodontitis lesions

WANG Yan-qing; HU Ju-hua; LI Song; HU Shu-li; ZHANG Hong-yan

doi:10.15956/j.cnki.chin.j.conserv.dent.2015.02.003

Abstract：AIM:To examine the expression of cyclophilin A (CypA)in human periapical lesions and to investigate its relationship with the clinical symptoms of periapical periodontitis.METHODS:Samples of human api-cal periodontitis lesions of 83 cases were subjected to immunohistochemical examination for CypA and RANKL expres-sion.Six 6 healthy dental pulp samples obtained from orthodontic patients served as the controls.The integrated aver-age optical density value (A)of CypA and RANKL was measured by immunohistochemical analysis for all the samples. Differences in CypA and RANKL expression between different clinical presentations were subsequently analyzed by t -test.RESULTS:CypA postitive cells and RANKL positive cells were both detected in all periapical lesion tissues, and the expression of CypA was significantly higher in periapical lesions than in normal tissues (P <0.05).Epithelial cells,lymphoid cells and endothelial-like cells showed CypA positive in the lesions.The expression of RANKL in cyst group were higher than in granuloma group (Z =4.48,P <0.05),while the expression of CypA in granuloma group were higher than in cyst group (Z =4.48,P <0.05).The average (A)value of CypA positive sites in periapical le-sions with clinical symptoms was higher than that without clinical symptoms(77.24 versus 39.18,P <0.05).Positive correlation was observed between CypA expression and lesion size(r =0.462,P <0.05).Moreover,positive correla-tion between RANKL expression and lesion size was observed (r =0.417,P <0.05).CONCLUSION:CypA might be involved in the inflammatory response and bone resorption,and associated with periapical lesion pathogenesis.