The effects of cell-cell interaction of dental papilla cells and dental follicle cells on cell pluripotency
LIU Lu
PENG Zheng-jun
WEI Xi
XU Zhe-zhen
Abstract：AIM:To investigate the effect of cell-cell interaction of dental papilla cells (DPCs)and dental follicle cells (DFCs)on the pluripotency of the cells.METHODS:An in vitro cell-cell co-culture system of DPCs and DFCs was established.Cell growth,cell senescence and the expression of Oct-4,Sox2 and c-Myc were investiga-ted by cell counting,β-gal staining and immunohistochemical staining.The odontogenic differentiation capability of DPCs and DFCs was evaluated by ALP activity assay after 0,7,14,and 21 d of osteogenic induction.RESULTS:Under co-culture condition,cell growth of DPCs and DFCs was promoted,and cell senescence of DPCs and DFCs was inhibited.Expression of Oct-4,Sox2 and c-Myc was significantly elevated in both cells on day 5 after coculture.ALP activity was significantly upregulated in both cells after 14d and 21d of odontogenic induction.CONCLUSION:Opti-mized microenvironment with cell-cell communication may enhance the pluripotency potential of DPCs and DFCs.
Keywords：dental papilla cells (DPCs)dental follicle cells (DFCs)microenvironmentpluripotency
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 63-67 )