The effects of Schisandrin A on the virulence factors of enterococcus faecalis
CUI Hong
XU Ying
WU Qing
Abstract：AIM:To investigate the antibacterial effect of schisandrin A on Enterococcus faecalis biofilm and on the expression of the virulence factors.METHODS:Enterococcus faecalis biofilm was cultured in vitro.The Entero-coccus faecalis biofilm was co-cultured with schisandrin A at 31.25,62.5,125,250 and 500 μg/mL respectively for 12 h,MTT assay was conducted to determine the viable of Enterococcus faecalis in the biofilm.Enterococcus faecalis biofilm was co-cultured with schisandrin A at 125,250,and 500μg/mL for 24 h,then srtC,esp and ebpR mRNA was examined by RT-PCR.RESUITS:Schisandrin A showed a dose-dependent inhibitory effect on Enterococcus faecalis wiability(P<0.05).The highest inhibition was achieved at 500 μg/mL of schisandrin A.Moreover,schisandrin A decreased srtC,esp and ebpR mRNA in a concentration dependent manner(P<0.05 ).Schisandrin A at 500 μg/mL completely inhibited the mRNA Expression of srtC,esp,and ebpR.CONCLUSION:Schisandrin A can inhibit the proliferation of Enterococcus faecalis and the expression of virulence factors srtC,esp,and ebpR mRNA of Enterococcus faecalis.
Keywords：schisandrin aEnterococcus faecalisbiofilmvirulence factorRT-PCR
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 24-27 )