Preparation of PLGA and chitosan nanoparticles and test of their cytotoxicity

XUE Yan-xiang; ZHAN Xue-ling; LIU Ying; SHEN Ren-ze; JIN Bin-yu; GAO Jie

Abstract：AIM:To prepare PLGA and chitosan ( CS) nanoparticles and to evaluate their cytotoxicity to periodontal ligament cells ( PDLCs) .METHODS:PLGA and CS nanoparticles were prepared by emulsion solvent e-vaporation method cross-linking of CS with sodium tripolyphosphate respectively.The nanoparticles were characterized by Zetasizer 3000HS and transmission electron microscope.Passage 3 -5 PDLCs were cultured with PLGA and CS nanoparticles at 0(the control), 0.5 mg/mL, 1 mg/mL and 2 mg/mL respectively.The PDLCs were subjected to MTT assay at 24, 48, 72 h of culture.RESULTS:PLGA nanoparticles were uniform and spherical, and the mean particle size and dispersive coefficient were 188.10 ±2.86 nm and 0.55 ±0.04, respectively.CS nanoparticles were relatively uniform and aspheric, the mean particle size and dispersive coefficient were 250.97 ±30.86 nm and 0.50 ±0.07, re-spectively.The OD values of 2 mg/mL PLGA group and 1 mg/mL CS group were lower than that of the control (P<0.05) at 48 h of culture.At other times points, no significant difference was found among different groups (p>0.05).The relative growth rate of PDLCs in all groups was all higher than 85%.The toxicity grading of all groups was 0 or 1.CONCLUSION:PLGA and CS nanoparticles are nontoxic to PDLCs, according to ISO10993-5.