A histomorphological observation of cellular cementum devolopment in BALB/c mice
YU Xi-jiao
DU Yan-mei
JIANG Huan
Du Yi
Abstract：AIM:To observe the histological and cellular activity changes during cellular cementum develop-ment in BALB/c mice.METHODS:Post-natal 15, 19 and 25d BALB/c mice (8 in each group) were sacrificed and cellular cementum of mandibular first molar was taken.Cytokeratin 14 ( CK14 ) expression in epithelial cells of Hertwig′s epithelial root sheath ( HERS) was examined by PV two-step immunohistological method.The morphology and distribution of epithelial cells and cellular cementum were examined by light and transmission electron microscope ( TEM) .Apoptosis was identified by the terminal deoxy-transferase ( TdT)-mediated dUTP-biotin nick end labeling ( TUNEL) method.RESULTS:During cellular cementogenesis, some cells were incorporated in the cementum and positive CK14 expression can be observed.Following the disintegration of HERS, cementum matrix was rapidly deposi-ted around cementoblasts.Epithelial cells and cementoblasts on the root surface were embedded as cementocytes. CONCLUSION:Epithelial cells from disintegrated HERS may directly participate in cellular cementogenesis.
Keywords：hertwig's epithelial root sheath( HERS)cellular cementumCK14development
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 701-704 )