Construction of lentiviral miR101 expression system and the role of miR101 in the regulation of PLAP-1 expression during osteogenic differentiation of periodontal ligament cells
LI Chang-xia
LUE Chang-yi
LI Chun-lei
YUE Jing
HUANG Xin
WU Bu-ling
Abstract：AIM:To investigate whether miR101 regulates the gene expression of PLAP-1 in human perio-dontal ligament cells ( PDLCs) .METHODS:Overexpression and downexpression systems of miR101 were constructed with pLVX-shRNA1 system.PDLCs were cultured with osteogenesis induction medium and infected by the miR101 overexpression and downexpression systems respectively.Western blot was performed to confirm the effects of these constructs on PLAP-1 gene expression.RESULTS:Constructs of lentiviral-miR101 were obtained.Overexpression of miR101 reduced the expression level of PLAP-1.Downexpression of miR101 increased the expression level of PLAP-1.CONCLUSION:miR101 regulates PLAP-1 expression during osteogenic differentiation of PDLCs, sugges-ting that miR101 may play important roles in the osteogenic differentiation of PDLCs.
Keywords：PLAP-1periodontal ligament cellsosteogenic differentiationmiR101
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 696-700 )