The effects of gallnut water extraction on the alkaline phosphatase expression and mineralization of human dental pulp cells
LU Jing
XU Xing-xing
LI Xing
JIA Zhen
LI Yan
MI Xiu-kui
Abstract：AIM:To study the effects of gallnut water extraction on the alkaline phosphatase( ALP) activity and mineralization of human dental pulp cells( hDPCs) .METHODS:hDPCs were cultured with gallnut water extrac-tion at 2.5,5,10, 20 and 40μg/mL respectively.ALP expression of the cells was examined by immunocytochemistry after 48 hour culture.According to the ALP expression, the cells were treated by gallnut water extraction at 10μg/mL and 20μg/mL respectively for 30 days and Von Kossa staining was performed to detect mineralization.RESULTS:The extraction at 2.5μg/mL and 5μg/mL had no significant effect on ALP activity of the cells, while at 10, 20 and 40μg/mL inhibited ALP expression of the cells.Both 10μg/mL and 20μg/mL gallnut water extraction could promote mineralization of hDPCs after continuous cultivation for 30 days.CONCLUSION:Gallnut water extraction can change the ALP activity and mineralization of human dental pulp cells.
Keywords：gallnut water extractculture in vitrohuman dental pulp cell( hDPCs)ALPmineralization
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 692-695 )