Proliferation and osteogenic differentiation of tooth germ cells with different diameters
LI Jun-jun
YAN Ming
WU Jin-tao
JING Shuang-lin
WEI Xin
ZHANG Guang-dong
YU Jin-hua
Abstract：AIM: To compare the proliferation and osteogenic differentiation of rat tooth germ cells ( rT-GCs) with different diameters.METHODS:rTGCs were isolated enzymatically from Sprague-Dawley rats and divided into larger and smaller populations using a 13 μm cell sieve.MTT assay was performed to evaluate the cell prolifera-tion.Alkaline phosphotase ( ALP) kits, alizarin red staining and quantitative PCR were used to investigate the osteo-genic differentiation capacity of the cells.RESULTS: The larger rTGCs( rTGCs-Large) showed greater proliferation rate than the smaller counterparts ( rTGCs-Small) .However, rTGCs-Small group presented higher ALP activity at 3 and 7 d after culture and more calcified nodules than rTGCs-Large group at 14 d after culture.Moreover, rTGCs-Small group expressed stronger osteogenic markers Ocn, Osx and Runx2 than rTGCs-Large group.CONCLUSION:rTGCs-Small have lower proliferation capacity but stronger osteogenic differentiation potential than rTGCs-Large group.
Keywords：proliferationdifferentiationtooth germ cellsdiameter
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 687-691 )