Effect of CeCl3 on the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro
HU Ying
JIANG Huan
DU Yi
Abstract：AIM:To investigate the effects of CeCl3 on the osteogenic differentiation of bone marrow mesen-chymal stem cells (BMSCs).METHODS:BMSCs were obtained from porcine bone marrow aspiration and cultured inα-MEM medium with 10% FBS.The cells of third passage were exposed to inducing medium with or without CeCl3 . The alkaline phosphatase (ALP)activity was measured.The gene expression of bone-related protein Runx2,BSP and OCN was analyzed by real-time RT-PCR.RESULTS:In the CeCl3 treated cells ALP activity,the gene expression level of bone-related marker Runx2,BSP and OCN were obviously higher than those in the control cells.CONCLU-SION:CeCl3 may stimulate the osteogenic differentiation of BMSCs.
Keywords：CeCl3mesenchymal stem cellsosteogenic differentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 639-641,676 )