The biological characteristics of electrospun ibuprofen-loaded PLGA/PLA nanofibrous GTR membrane:An in vitro study
HE Miao
ZHANG Er-shuai
WU Zhen-zhen
LIU Jun-yi
WANG Yong-lan
YAO Fang-lian
Abstract：AIM:To investigate the biological performance of ibuprofen(IPF)-loaded electrospun poly lac-tic-co-glycolic acid (PLGA)/poly lactic acid (PLA)nanofibrous guided tissue regeneration membrane.METH-ODS:PLA nanofibrous membranes and IPF(at 5%,10% and 15% respectively)-loaded PLGA/PLA (IPF-PL-GA/PLA)nanofibrous membranes were synthesized using an electrospinning technique.The morphology of the nanofi-bers was observed under SEM.Human gingival fibroblasts were seeded on IPF -PLGA/PLA membranes and HGFs proliferation was assessed with MTT assay.Drug entrapment efficiency was determined and drug release profile was tested.RESULTS:The diameters of the nanofibers were 300 nm -5 μm,and the IPF -loaded nanofibers were smooth,uniform and continuous.The majority of EMT-6 cells adhered on the surface of PLA membranes,while almost no cells appeared in the membranes.MTT assay showed that the number of EMT-6 cells of the experimental group was not significantly different from that of the control group after 1,3,5 and 7 day culture(P>0.05).Ibuprofen en-trapment efficiencies were above 85%for all the IPF-loaded membranes.The release of ibuprofen at 5%、10%、15%wrapped in fiber was 30%,60%and 85%respectively in 7 days and the release was going on for 10 -28 days.CON-CLUSION:IPF-PLGA/PLA nanofibrous membranes are biocompatible with excellent barrier function for cells and slow drug release.
Keywords：ibuprofenpoly lactic-co-glycolic acidpoly lactic acidelectrospinning techniqueguided tissue regeneration membranedrug-loaded
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 634-638,648 )