Dentin barrier cytotoxicity test of dental restorative materials
LI Ya-ping
ZHAO Xin-yi
LI Shi-bao
TANG Li-hui
GUI Ya-jie
Abstract：AIM:To compare cell reaction to five common dental filling materials tested with an indirect contact method equipped with dentin barrier and a conventional extraction culture method.METHODS:Dentin discs with 0.5mm in thickness were cut from the buccal inner dentin of freshly extracted molars and were determined for hy-draulic permeability.The discs with similar permeability were used as barrier to prepare device for cytotoxicity test in vitro.Cytotoxicity of composite resin,ZOE,GIC,Ca(OH)2 and ZPC cement,was tested using both this device and conventional extraction culture method with MTT assay.RESULTS:Relative cell viability (RCV,%)obtained with dentin barrier for the five filling materials were:81.8 ±3.7,89.1 ±3.6,96.4 ±2.8,96.2 ±1.8 and 74.5 ±2.8 re-spectively.The RCV(%)obtained with extraction culture method were 72.9 ±4.3,35.6 ±5.2,91.5 ±1.3,90.2 ± 2.6 and 59.3 ±2.6 respectively.CONCLUSION:Dentin barrier reduced the toxicity of filling material to cells,and the results from dentin barrier method are more reliable in reflecting the in vivo toxicity of filling materials.
Keywords：dental filling materialspulpcytotoxicitydentin barrier
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 625-629 )