Molecular identification of the microorganisms in well-filled-root canal associated with endodontic treatment failure
ZHANG Fu-hua
YAO Li-li
ZHANG Jian-feng
HE Ai-min
Abstract：AIM:To assess microbial diversity in root-well-filled teeth associated with failed endodontic treatment.METHODS:DNA was extracted from 20 root-canal-treated teeth with post-treatment apical periodontitis, the 16S rRNA of all present bacteria was amplified by PCR,followed by cloning and sequencing of the 16S rRNA ampl-icons.RESULTS:Bacteria were present in all cases.15 phylotypes belonging to 4 phyla were found in 423 clones;a mean of 2.7 phylotypes were identified in each sample (range 1 ~6).Enterococcus faecali (60%,12/20)and pep-tostreptoccus micros (40%,8/20)were the prevalent species.The other 13 species were found in a few cases respec-tively,rummeliibacillus,exiguobacterium aurantiacum,pseudomonas veronii,phocaeicola abscessus were respectively de-tected in one patient.CONCLUSION:A high diversity of the microbial flora is associated with endodontic treatment failures.Enterococcus faecali is the most frequently identified species by 16S rRNA gene clone library analysis.
Keywords：16S ribosomal RNAbacteriaendodontic treatment failure
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 586-591 )