The effects of nicotine on prostaglandins-2 expression in periodontal tissues during orthodontic tooth movement in rats
PAN Xu
ZU Li-hu-ma.A Re-fu-jiang
QIAN Ya-jing
HU Ming-hua
NIE Jing
MI Cong-bo
Abstract：AIM To investigate the effects of nicotine on prostaglandins-2 (PGE 2)expression in periodon-tal tissues during orthodontic tooth movement in rats.METHODS:100 SD rats were randomly divided into 4 groups (n=25).Orthodontic force of 50g was placed on one side of the first maxillary molar of all rats for mesial movement. During tooth movement,the rats in experimental group were intraperitioneally injected with 0.5 mg/kg,0.75 mg/kg or 1 mg/kg nicotine tartrate solution daily.The rats in control group was injected with 0.1 ml saline for each.5 rats in each group were sacrificed at 1,3,5,7 and 14 days respectively after operation.The maxillary tissue blocks were processed and the periodontal tissues were stained with HE.The number of osteoclasts was calculated and PGE-2 in periodontal tissues were examined by immunohistochemical staining.RESULTS:Nicotine dose-dependently increased the number of osteoclasts(P<0.05)and PGE-2 expression(P<0.05)in periodontal tissues,and the effects reached maximum at day 5 of tooth movement.CONCLUSION:Nicotine can dose dependently increase the expression of PGE-2 and the osteoclast number in periodontal tissues during tooth movement.
Keywords：nicotineorthodontic forcetooth movementPGE-2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 572-576 )