Effects of BMP-2 on the proliferation and odontoblast differentiation of dental pulp cells
QIN Wei
GAO Xian-ling
LIN Zheng-mei
Abstract：AIM:To investigate the effects of bone morphogenetic protein-2 (BMP-2)on the biologic ac-tivity of human dental pulp cells (DPCs)in vitro.METHODS:Human DPCs were in vitro cultured .The cells of passage 4 were cultured in normal medium (for cell proliferation assay)or osteogenic medium (for osteogenic differen-tiation assay)containing BMP-2 at 50,100,and 200 ng/mL,respectively.while medium without BMP-2 served as the control.The cell proliferation was examined by MTT method.ALP activity,the quantity of calcified nodules and mRNA expression of DSPP and DMP-1 were evaluated and quantified by RT-PCR.RESULTS:BMP-2 did not show effect on the proliferation of DPCs.BMP-2 at all tested concentrations increased ALP activity (P<0.05),the calcified nodules(P<0.05)and mRNA levels of DSPP and DMP-1 in DPCs (P<0.05)in a dose-and time-dependent manner. CONCLUSION:BMP-2 can promote odontoblast differentiation of DPCs,but has no significant effect on the prolifera-tion of DPCs.
Keywords：bone morphogenetic protein (BMP)dental pulp cellsproliferationdifferentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 568-571 )