Calcitonin regulates periodontal ligament cell proliferation and collagen synthesis via TGF-βupregulation
DAI Ying
YE Qing
WEI Yi-bo
CAO Zhi-zhong
Abstract：AIM:To investigate the effect of calcitonin ( CT) on the proliferation and collagen synthesis of periodontal ligament cells (PDLCs), and to explore the possible signal pathway involved .METHODS: CT gene re-combinant adenoviruses ( Ad.CT) was constructed and PDLCs were transfected with Ad .CT alone or co-treated with LY2109761.Cells transfected with Ad.Null served as the controls.Flow cytometry was used to observe cell cycle of the PDLCs.The mRNA and protein expression of CT, collagen (type I, III and IV) and transforming growth factor-β( TGF-β) were measured by RT-PCR and western blot .RESULTS: CT mRNA and protein expression in Ad .CT group was significantly higher than that in Ad .Null group (P<0.05).That the proportion of S/G2/M phase cells in Ad.CT group was significantly higher than that in Ad .Null group (P<0.05).Collagen synthesis and TGF-βexpres-sion were significantly higher in Ad.CT group than those in Ad.Null group (P<0.01).Ad.CT and LY2109761 co-treated PDLCs showed significantly lower percentage of S /G2/M phase cells and than Ad .CT treated cells .CONCLU-SION:CT might promote PDLCs proliferation and increase collagen expression through TGF -βpathway .
Keywords：calcitoninHuman periodontal ligament cellstransforming growth factor-βcell proliferationcol-lagen synthesis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 505-509,521 )