The effectc of autologous platelet-rich plasma gel precipitation liquid on the migration of marrow mesenchymal stem cell migration
XIE Man-Jing
CHEN Wen-xia
HUANG Yang
LI Kang-jing
Abstract：AIM:To investigate the effect of Platelet-rich plasma gel(PRG)precipitation liquid(PL)on the migration of bone marrow stem cells(BMSCs)in vitro.METHODS:Canine BMSCs was obtained by adherence separation method.PRGPL was prepared by duplicatese centrifugation and diluted with DMEM to a volume fraction of 1%,5%,10%,20%and 50%solution,BMSCs were cultured with PRGPL at the various concentrations for 24 h at 0(control)and 5% for 12 h,24 h and 36 h respectively.The migration of BMSCs was examined by migration assay with transwell migration model.RESULTS:PRGPL at 1%,5%,10% and 20% promoted the migration of BMSCs (P<0.05);while at the concentration of 50%restrained the migration(P<0.05).PRGPL at 5%showed the maxi-mum effect and time-dependeant migration increase (between 12 h and 24 h culture,P<0.05 ),between 24 h and 36 h culture,P>0.05).CONCLUSION:The platelet-rich plasma gel precipitation liquid at concentrations of 1%, 5%,10%,20% can promote the migration of BMSCs,this migration effect is in a time-dependent manner.
Keywords：platelet rich plasma gel precipitation liquidbone marrow mesenchymal stem cellscell migration
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 446-450 )