Effects of nicotine on the proliferation and interleukin-8 expression of human gingival fibroblasts
SUN Hong-tao
KONG Fan-zhi
GAO Jin-fu
Abstract：AIM:To investigate the effects of nicotine on the proliferation and IL-8 expression of human gingival fibroblasts(HGFs).METHODS:HGFs were primarily cultured and identified.MTT method was used to ex-amine the viability of the cells stimulated by nicotine at the concentrations of 10-2000 μg/mL respectively for 72 h. ELISA was used to detect IL-8 concentration in the culture supernatant after 72 h stimulation .RESULTS:Nicotine dose-dependently inhibited the proliferation of HGFs (P<0.05).10-500μg/mL nicotine stimulated IL-8 secretion in HGFs,100μg/mL nicotine showed the most significant stimulating effect (P<0.05 ).CONCLUSION:Nicotine can inhibit the proliferation of HGFs and increase IL-8 production by HGFs,indicating that nicotine may play a role in the progression of periodontitis in tobacco users.
Keywords：human gingival fibroblasts(HGFs)IL-8nicotineperiodontal diseasemethyl thiazolyl tetrazoli-um(MTT)enzyme-linked immunosorbent assay(ELISA)
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 390-392 )