Effects of hypoxia preconditioning and AMPK overexpression on proliferative inhibition of dental pulp cells by hydrogen perioxide
ZHANG Ping
LI Cheng
YU Bo
Abstract：AIM:To investigate the effects of hypoxia preconditioning and AMP-activated protein kinase (AMPK)overexpression on proliferative inhibition of dental pulp cells by hydrogen perioxide(H2O2).METHODS:The expression of AMPK mRNA in dental pulp cells was analyzed by qRT-PCR after the cells had been exposed to nor-moxia (950 mL/L O2 )or hypoxia (10 mL/L O2 )for 12 h.The cells were then cocultured with 0.1 mmol/L H2 O2 for 4 h and cell proliferation was evaluated by MTT assay.Furthermore,the cells were transfected with AMPK overexpres-sion vector and specific siRNA respectively.Then transfected cells were cocultured with 0.1 mmol/L H2 O2 for 4 h,the proliferation of the cells was evaluated by MTT assay.RESULTS:Hypoxia preconditioning upregulated AMPK mRNA levels in dental pulp cells and ameliorated proliferative inhibition by H2 O2 (P<0.05 ).The cells transfected with AMPK overexpression vector attained higher proliferative rate than the controls or those transfected with siRNA after challenging with H2 O2 .However,AMPK knockdown significantly inhibited proliferation of the cells (P <0.05 ). CONCLUSION:Hypoxia preconditioning and AMPK up-regulation may confer resistance to oxidation,hence de-crease the proliferation inhibition induced by H2 O2 in dental pulp cells.
Keywords：hypoxia preconditioningAMP-activated protein kinase(AMPK)dental pulp cellhydrogen peri-oxideproliferation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 385-389,418 )