The effect of cysteine protease hydrolysis on the pigment reabsorption of residual protein film
ZUO Qi-liang
ZHANG Yi
YAO Jiang-wu
Abstract：AIM:To determine the pigment reabsorption ability of protein/pigment complex layer and pro-tein monolayer after being hydrolyzed respectively by three kinds of cysteine proteases (CPs).METHODS:The de-phosphorylated bovine β-casein (Dβ-CN)/theaflavin (TF)complex layer and Dβ-CN monolayer were established self-assembly on a sensor surface of quartz crystal microbalance with dissipation (QCM-D),respectively.After both categories of layers had been hydrolyzed by cysteine protease papain,bomelian,and ficin respectively,the thickness of residual layers and the mass of TF re-adsorbed on the sufaces were measured.The data were analyzed by one-way AVNOA and SNK-q test,then were fitted by a nonlinear regression method.RESULTS:The thickness of Dβ-CN/TF complex layer and Dβ-CN monolayer were decreased after treatment with the 3 kinds of CPs (P<0.05).The mass of re-adsorpted TF on the surface of residual layer was reduced along with the decreases of layer thickness (P<0.05), the effects whowed a positive nonlinear corelation.CONCLUSION:Hydrolyzation by CPs can decrease the affinity be-tween TF and residual complex layer or monolayer.CPs may be used for proteinaceous tooth stain removal and preven-tion.
Keywords：theaflavin(TF)cysteine proteases(CPs)dephosphorylated bovine β-casein(Dβ-CN)quartz crystal microbalance with dissipation(QCM-D)
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 381-384 )