The effects of developing apical complex cells conditioned medium on the proliferation and differentiation of ADSCs
ZHENG Gui-ting
XU Yan
ZHAO Xuan
SHEN Ji-long
Abstract：AIM:To study the effects of the developing apical complex (DAC)cells conditioned medium (DACCM)on the proliferation and differentiation of adipose tissue-derived stem cells (ADSCs).METHODS:DAC cells,separated from the 20 d postnatal SD rat,were cultured by tissue block combined with enzyme digestion method. DACs was identified by HE staining and immumohistochemical staining.DACCM was collected and prepared by filtra-tion and none-filtration (DACCM-F and DACCM-UF)respectively.The proliferation of ADSCs was detected by MTT assay.The mRNA and protein expression of alkaline phosphatase (ALP)were detected by RT-PCR and ALP detection Kit respectively.SPASS13.0 was used for data analysis.RESULTS:DAC cells were obtained from the developing roots of the SD rat.The cells were CK-14 and vimentin positive.The proliferation,ALP mRNA and protein expression of ADSCs cultured in DACCM-UF exceeded those of the cells cultured in α-MEM or DACCM-F(P<0.05 )CON-CLUSION:DACCM-UF may stimulate the proliferation and differentiation of ADSCs.
Keywords：developing apical complex (DAC)adipose tissue-derived stem cells (ADSCs)conditioned medi-umproliferationdifferentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 331-337 )