Effects of PRP on the differentiation of human dental pulp stem cells/endothelial progenitor cells in co-culture
LI Xin-zhu
WU Bu-ling
HOU Jin
WU Jing-yi
CHEN Ting
LUO Ao-xiang
Abstract：AIM:To explore the effects of PRP and the interactions of human dental pulp stem cells (hDP-SCs)with endothelial progenitor cells (EPCs)in the differentiation of hDPSCs into ontoblasts in vitro.METHODS:hDPSCs and EPCs were cultured in vitro and were divided into 6 groups:EPCs+10%FBS,EPCs+10%PRP,hDP-SCs+10%FBS,hDPSCs+10%PRP,hDPSCs+EPCs+10%FBS and hDPSCs+EPCs+10%PRP.The proliferation activity of the cells was detected by MTT assay.the mRNA expression of ALP,DMP-1 and DSPP was examined by qRT-PCR.RESULTS:The proliferation ability of the cells in the cultures with PRP was higher than that with FBS af-ter 5 or 9 days of culture(P<0.05).After 7 or 14 days of culture the expression of ALP,DMP-1 and DSPP in the cultures with PRP was higher than that with FBS (P<0.05)and was higher in co-cultures than in respective cultures (P<0.05).CONCLUSION:PRP can promote the proliferation of hDPSCs and can the promote the differentia of the cells into ontoblasts,especially in co-culture.
Keywords：platelet-rich plasmahuman dental pulp stem cellsendothelial progenitor cellsco-cultureodon-togenic differentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 317-322 )