Effects of free amino acids on Streptococci mutans proliferation and Bioflim formation
ZHANG Luo-dan
LING Jun-qi
TONG Zhong-chun
Abstract：AIM:To investigate the effects of free amino acids on the proliferation and biofilm formation of Streptococcus mutans (S.mutans).METHODS:S.mutans UA159 was cultured with 20 D-or L-free amino acids at 40 mmol/L recpectively.Time-kill assay was used to evaluate the proliferation of S.mutans,and the viable bacterial cells were counted by plate count at 4,8,12 and 24 h respectively after treatment.Crystal violet staining was used to observe the biofilm formation of S.mutans.RESULTS:D-Cys,D-Arg,L-Lys decreased S.mutans proliferation (P<0.05 );D-or L-enantiomers of Asp and Glu inhibited the proliferation of S.mutans (P<0.05 );D-Phe, L-His,L-Cys decreased S.mutans proliferation at the early stage;D-or L-Ser,Tyr and L-Val delayed S.mutans proliferation at the exponential phase.D-or L-enantiomers of Cys,Asp,Glu,D-Arg,D-Trp inhibited the formation of S.mutans biofilm.CONCLUSION:Some free amino acids can inhibite the proliferation and biofilm formation of S.mutans.
Keywords：S.mutansfree Amino acidsbiofilm
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 313-316 )