Effect of autophagy on the expression of stemness marker genes in mouse bone marrow mesenchymal stem cells
LUO Ke-fu
LIU Wen-Jia
CHEN Ji-hua
JIN Yan
Abstract：AIM:To study the effect of autophagy on the expression of stemness marker gene Nanog,Oct-4 and Sox-Ⅱ in mouse bone marrow mesenchymal stem cells(BMSCs).METHODS:Mouse BMMSCs were isolated, cultured and identified by flow cytometry analysis.Autophagy of BMSCs was induced by rapamycin or inhibited by siR-NA targeting beclin-1 (si-beclin 1 ),the level of autophagy of BMMSCs and the expression of Nanog,Oct-4 and Sox-Ⅱ were examined by Real Time PCR (RT-PCR).RESULTS:The cultured BMMSCs expressed CD90.2 (97.4%),CD29 (97.6%),SCA-1 (83.5%),CD31(2.9%),CD146 (2.4%)and CD11b (5.6%).Treatment with rapamycin increased Nanog,Oct-4 and Sox-Ⅱexpression (P<0.05),whereas,si-beclin 1 decreased the auto-phagy acticity and down-regulated Nanog,Oct-4 and Sox-Ⅱ expression (P<0.05)of BMMSCs.CONCLUSION:Increasing autophagy activity of BMMSCs may be favourable for the cells to maintain the stemness.
Keywords：autophagybone marrow mesenchymal stem cellsstemness marker genes
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 251-257 )