Preliminary evaluation of the biological safety of RF Zirconia

Abstract：AIM:To preliminarily evaluate the biological safety of RF Zirconia. METHODS:According to IS07405 - 1997 (EGB) 16886-1997 standards, acute systemic toxicity test, vitro cytotoxicity - test and oral mucosal membrane stimulation test were carried out in Kunming mice and Mesocricetus auratus. RESULTS:In acute systematic toxicity tests all of the tested mice survived . No statistic difference was found in terms of body weight. By HE staining, no abnormality was observed in kidney and liver tissues. In cytotoxicity test, OD-values of each test group were similar to that of the negative control and significantly lower than that of the positive control group (P > 0.05). The cytotoxicity was grade 0 according to the test standard. The grade of oral mucous membrane stimulation test in Mesocricetus auratus was all less than 4, which is considered to be no irritation to mucous membrane according to medical standard. CONCLUSION:RF was preliminarily proved to be safe for dental application.