human Periodontal Ligament fibroblasts
GE Xin
WANG Yong
LIU Ying-feng
WANG Xiao-jing
Abstract：AIM:To examine whether human periodontal ligament fibroblasts (hPDLF) express Choline acetyltransferase (ChAT), a non-neuronal acetylcholine system substance, and to observe the effects of nicotine on of ChAT in hPDLF. METHODS:Expression of ChAT in hPDLF was detected by immunohistochemistry (IHC) at protein level and by RT-PCR at mRNA level. The effects of nicotine on ChAT was observed by treating hPDLF with nicotine (10-12mol/L) for 24 h. RESULTS:Positive staining of ChAT was detected by IHC in the cytoplasm of hPDLF. mRNA expression of ChAT in hPDLF was confirmed by RT-PCR. Nicotine treatment significantly increased both the mRNA and protein expression of ChAT in hPDLF (P<0.05). CONCLUSION:hPDLF expresses ChAT and nicotine can up - regulate ChAT expression at mRNA and protein levels in hPDLF.
Keywords：non-neuronal acetylcholine systemcholine acetyltransferasehuman periodontal ligament fibroblastsperiodontitisnicotine
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)