In vitro study on the odontogenic differentiation potentiality of bone marrow mesenchymal stem cells
DONG Jin-shan
DUAN Qing-yue
WEN Jun
JIN Chao-wei
HAO Jing-hui
LU Qun
Abstract：AIM:To explore the possibility of bone marrow mesenchymal stem cells (BMSCs)differentiating into odontoblast-like cells. METHODS:BMSCs were isolated from two-week Sprague-Dawley rats and cultured in tooth germ cells conditioned medium (TGC-CM). After 7 d induction, expression of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), and specific markers of odontoblasts, were observed by immunofluorescence staining and RT-PCR. RESULTS:After 7 days induction in TGC-CM, positive staining of DSP and DMP - 1 were detected in BMSCs by immunofluorescence staining. RT - PCR demonstrated mRNA expression of dentin sialophosphoprotein (DSPP) and DMP-1 by BMSCs. CONCLUSION:Rat BMSCs induced by TGC-CM could differentiate into odonto-blast - like cells.
Keywords：tooth germ cell conditioned mediumbone marrow mesenchymal stem cellsodontogenic differentiation
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)