Analysis of human odontogenic ameloblast-asssociated promoter activity regions

HAN Ting-ting; LIU Xiao-ying; HAO Jian-zhong; GAO Yu-guang

Abstract：AIM:To construct luciferase report gene vectors with different promoter segments of human odontogenic ameloblast-asssociated protein (ODAM) , and to compare the luciferase activities of different length segments of human ODAM in ameloblast and Hela cells so as to provide information for determining transcription regulation district of promoter sequence in further study. METHODS:The different-length desired promoter segments were obtained by PCR method, and cloned into luciferase report gene vectors pGL3-Basic. The construction was transiently transfected into ameloblasts and Hela cells. Sussequently, the transcriptional regulatory capacity of the promoter segments was analysed by luciferase activity. RESULTS:The different-length promoter segments of ODAM gene was obtained. Different-length promoter recombinant luciferase reporter vectors were constructed successfully by cutting with two different restriction enzymes. Different-length promoters showed different activities in different cells. The specific transcriptional regulatory district were -333~~ -195 and -195~~ -96 regions. CONCLUSION:The transcriptional activity regions of ODAM gene promoter were determined, which will provide useful information for further studying the transcriptional regulatory characteristics of ODAM gene.