Methylglyoxal induces gene expression profile changes in human periodontal ligament fibroblasts
LIU Xue-wei
YANG Ke
ZHANG Jie
MU Hong
CUI Xiao-guang
SUN Juan
Abstract：AIM: To analyze the differential gene expression in human periodontal ligament fibroblasts (HPDLFs) induced by methylglyoxal (MG) through gene chip technology. METHODS: HPDLFs were isolated from healthy periodontal ligaments of subjects undergoing tooth extraction for orthodontic treatment. For differential gene expression study, HPDLFs were cultured in DMEM with or without designated MG for 24h. The differential gene expression in HPDLFs stimulated by MG was analyzed by gene chip technology. RFSULTS: Gene gene expression profile analysis showed that 5 genes were upregulated and 3 genes were downregulated when cells were treated with MG. Further analysis demonstrated that those genes were related to cell metabolism and cancer. CONCLUSION: MG has cytotoxic effects on HPDLFs and alter the gene expression of HPDLFs.
Keywords：methylglyoxal(MG)gene chiphuman periodontal ligament fibroblastsgene expression
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 135-138 )