Induced differentiation of ectomesenchymal stem cells to muscle cells
ZHOU Ze-yuan
YU Shu-xiang
SHI Jun-nan
ZHANG Jian-ping
JIN Yan
Abstract：AIM:To induce the differentiation of ectomesenchymal stem cells (EMSC) to muscle cells.METHODS: The EMSC were cultured in DMEM containing 5 μL/mL Dimethyl sulphoxide (DMSO). Reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry were used to identify the characteristics of the cells. The rat fibroblast was used as control. RESULTS: RT-PCR showed that after 7d induction, EMSCs expressed Myod mRNA. After 14d induction positive desmin staining was detect in EMSCs by IHC. The controll rat fibroblast did not express Myod mRNA and desmin after induction. CONCLUSION: The results indicated that the EMSC can be differentiated into muscle cells by the action of DMSO.
Keywords：ectomesenchymal stem cellsneural crest stem cellsplasticitytissue engineering
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 123-126 )