Affects of Sr-HA with different concentrations of strontium on biologic behaviour of osteoblast
WANG Wei
ZHANG Yu-mei
YAN Jun
HAN Yong
SUN Ji-feng
Abstract：AIM: To evaluate the response of osteoblast to strontium-sincorperated hydroxyapatite (Sr-HA) coatings prepared by micro-are oxidation (MAO) with different Sr concentrations. METHODS: MAO method was used to prepare coatings with different concentrations (2.22 %, 7.24 %, 15.15 % and 23.78 %) of strontium. Pri-mary osteohlasts were plated directly on the coated surface. Cell adhesion was examined 30, 60 and 90 min after cell plating. The cell proliferation was examined 1, 3, 7 d after plating by MTT method. Alkaline phosphatase (ALP) ac-tivity was assessed with ALP assay kit. RESULTS: 15.15 % and 23.78 % Sr-HA enhanced osteoblasts adhesion and proliferation, while 7.24 % Sr-HA increased the values of ALP activity after 7 d culture. CNCLUSION: Sr-HA coatings containing relatively high concentration of strontium promote osteoblasts attachment and proliferation, while coatings with relatively low concentration of strontium have stronger ALP increasing activity.
Keywords：strontiumhydroxyapatitemicro-arc oxidationosteoblastscoatings
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 71-75 )