Antimicrobial effects of tinidazole as root canal irrigant

ZHU Ming-yi; ZHU Ya-qin

Abstract：AIM: To investigate the antimicmbial effects of Tinidazole as root canal irrigant. METHODS: 100 single-rooted teeth with chronic periapical periodontitis were randomly divided into four groups, with 25 teeth in each group. The root canals were irrigated with hydrogen peroxide (HP) with tosylchloramide sodium (TS) (HP + TS), chlorhexindine (CH), tinidazole (TD), chlorhexindine with tinidazole (CH + TD), respectively. Before and after root canal irrigation, a paper point was placed in the lower 1/3 of root canal for 1 min to collect micro-bial sample. Immediately after sample collection, the paper points were placed on sodium thioglycollate solution and subsequently plated on blood agar plates and incubated in an anaerobic chamber for 48 h. Colony forming units (CFU) were counted and compared by ANOVA with post hoc Turkey tests. RESULTS: After root canal irrigation, CFU in all the 4 groups was significantly reduced (P < 0.05). The sequential CFU number reduction was HP + TS < CH < TD < CH + TD. The CFU in HP + TS group was significantly lower than the other 3 groups (P < 0.05). However, no signifi-cant difference was found between the TD and the CH + TD group (P > 0.05). CONCLUSION: Our results suggest that tinidazoh has stronger bacterialcidal effects and is a better candidate than chlorhexindine for root canal irrigation.