Growth and biofilm forming capability of a wild-type Streptococcus mutans strain and a fluorescence protein reporter gene-labeled Streptococcus mutans strain
WANG Wei
HU Nan
SU Ling-yun
NI Long-xing
Abstract：AIM: To compare the growth and biofilm forming capability of a wild-type Streptococcus mutans strain (UA140) and a fluorescence protein reporter gene-labeled Streptococcus mutans strain (UA140-mrfp). METH-ODS: The growth of UA140 and UA140-mrfp in BHI broth were measured at different time points at OD600. Biofilm amount and structure were observed with scanning electron microscope (SEM). Data were analyzed by " repeated meas-urement design ANOVA" with SPSS 10.0. RESULTS: There was no significant growth difference between the 2 strains although the OD 600 value of UA140-mrfp was slightly lower than that of the UA140 (P > 0.05). SEM demon-strated that there was no significant difference on the amount of biofilm between the two groups (P > 0.05). Further-more, the structure of the biofilms was similar. CONCLUSION: UA140-mrfp could be used as a substitute for UA140 in the research of biofilm formationce in vitro model systems.
Keywords：Streptococcus mutansfluorescence protein reporterwild-type straingrowth curvebiofilms
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 61-64 )