Differentiation and mineralization-inducing effect of polyvinyl alcohol on rat dental pulp cells
PANG Nan
ZOU Jing-cai
XUN Wen-xing
ZHANG Hong-mei
WANG Yan
QU Xiao-li
ZHANG Xiu-li
YANG Hai-zhen
YANG Xiang-ju
CHEN Jing-tao
CHEN Xiao-nan
Abstract：AIM:To investigate the mineralization inducing effect of polyvinyl alcohol(PVA)on dental pulp cells of SD rat.METHODS:Primary dental pulp cells of rat were cultured on non-coated(control),non-coated plus mineralization solution and PVD-coated 24 well culture plates in DMEM.The mineralization of the cells was determined by measuring the alkaline phosphatase(ALP)activity and by Alizarin Red S(ARS)mineralization assay at 1,4 and 7d after induction.RESULTS:At all time points,both PVA and mineralization inducing solution sisnificantly increased ALP activity and calcium sedimentation(P<0.01)of the cells.PVA showed much stronger differentiation inducing effect than the mineralization-inducing solution(P<0.01).CONCLUSION:PVA is able to induce the differentiation and mineralization of rat dental pulp cells and might be a suitable scaffold for the study of tissue engineering teeth.
Keywords：polyvinyl alcoholdental pulp cellsmineralizationAlizarin Red S mineralization assayALP
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 6-9 )