Influence of Chinese nutgall extraction on the inhibitory effect of lipopolysaccharide to ALP activity of human dental pulp cells
LAN Xue-song
TANG Rong-yin
Abstract：AIM:To observe the influence of Chinese nutgall extracts on the inhibitory effect of lipopolysaccharide to ALP activity of human dental pulp cells in different doses and action time. METHODS: Human dental pulp cells were cultivated by tissue explant. Human dental pulp cells of the fifth passage were cultured in DMEM with 100 μg/mL lipopolysaccharide, Chinese nutgall extracts(2.5 to 40μg/mL) and 20mL/L fetal bovine serium. ALP activity of cells was analyzed. One -way AN0VA(completely random design)and t -test were used in statistical analysis. RESULTS: Low -dose of Chinese nutgall extracts(≤10 μg/mL) can resist the inhibitory effect of lipopolysaccharide to ALP activity of human dental pulp cells significantly. The 5 μg/mL is the best. From the second day , the ALP activity of human plup cells incubated with 5μg/mL Chinese nutgall extracts and l00μg/mL lipopolysaccharide increased signicificantly in a time - dependent manner. The action of 20 μg/mL and 40 μg/mL is not obvious. CONCLUSION : Low - dose Chinese nutgall extracts can resist the inhibitory effect of lipopolysaccharide to ALP activity of human dental pulp cells.
Keywords：Chinese nutgalllipopolysaccharide(LPS)dental pulp cellsalkaline phosphatase(ALP)
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 673-676 )