Effects of Baicalin on Toll-like receptor 4 expression and tumor necrosis factor alpha secretion in human dental pulp cells induced by lipopolysaccharide
YU Miao
LEI Lei
TAN Wei-xia
ZHONG Bo
ZHANG Xiao-wei
WU Kan-kui
Abstract：AIM:To study the effects of baicalin on the expression of Toll - like receptor 4(TLR4) and the secretion of tumor necrosis factor alpha (TNF -a) in human dental pulp cells(HDPCs) induced by lipopolysaccharide (IPS) , and the mechanism of the effects. METHODS: HDPCs were primarily cultured in vitro and their phenotype was identified by immunohistochemcial staining. Flow Cytometry(FC) was used to analyse the expression of TLR4, and en zyme - labeled immunosorbent assay (ELISA) was used to measure the secretion of TNF - a. RESULTS: TLR4 and TNF - a were detected weakly in normal HDPCs , but their expessions significantly increased after being stimulated with LPS at 100|μg/mL( P < 0.01) . The expressions of TLR4 and TNF - a had no statistical differences between the control group and the group of 10μg/mL baicalin ( P > 0.05 ). Baicalin at the concentrations from 0.001 μg/mL to 20μg/mL could significantly inhibit the activity of TLR4 and TNF - a induced by LPS in a dose - dependent manner. Among the different baicalin concentration groups ,the differences of expression were statistically significant (P<0.01). CONCLUSION; LPS may participate in the inflammation of pulp tissue by TLR4. Baicalin may play a role in the treatment of pulpitis induced by LPS with the help of inhibiting the expression of TLR4 and secretion of TNF - a.
Keywords：baicalinlipopolysaccharidehuman dental pulp cellsToll-like receptor4TNF-a
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 632-636 )