Study on the induced nerve differentiation of the dental pulp stem cell and ectoblast mesenchyme stem cell in vitro
WANG Yi-jing
XU Li
JIN Yan
SHI Jun-nan
Abstract：AIM:To investigceate the multi-direction differentiation of the dental pulp stem cell(DPSC) and ectoblast mesenchyme stem cell(EMSC)by the neural induction in intro.METHODS:DPSC and EMSC were in duced in intro by inducing fluid containing forskolin and IBMX.Then the cells were identified with the morphologic change,cell immunohistochemistry and detection of NSE of neuron marker and GFAP.RESULTS:The morphology of the induced DPSC was similar to the neuron,and their ecphyma stretched out.Cytoplasm shrinking,cyton bulging and ecphyma stretching out around cell body were found in the EMSC.NSE was positive in the DPSC and negative in the EMSC with immunohistochemistry.A few of DPSCs were found to be positive expression of GFAP.GFAP was positive in the EMSC.CONCLUSION:DPSC and EMSC have the potentiality of differentiation to nerve cell by some kind of inducing conditions in intro.The induced terminal cells are different because of the change in the immigration process of the cell character.
Keywords：dental pulp stem cellsectoblast mesenchyme stem cellneuronneurogliocyte
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 616-618 )