Effect of interleukin-10 on the expression of monocyte chemotactic protein-1 in human dental follicle cells in vitro
QIAN Hong
SANG Han-fei
JIN Zuo-lin
DUAN Yin-zhong
HU Jing
Abstract：AIM:To study the effect of IL-10 on the expression of monocyte chemotactic protein-1(MCP-1)in human dental follicle cells(HDFCs).METHODS:HDFCs of the 5th passage were CO-cultured with 25ng/mL IL-10 for 0,1,3,6,9 and 12 h.The mRNA expression of MCP-1 Waft detected by RT-PCR.In the next experiment the cells were CO-cultured with 25ng/mL IL-10 for 0,2,4,6 and 8h.The protein expression of MCP-1 wag examined by ELISA.RESULTS:25ng/mL IL-10 down-regulated the mRNA expression of MCP-1 in 1-6h(P<0.05)and the protein expression of MCP-1 in 4-8h(P<0.05).CONCLUSION:IL-10 inhibits MCP-1 expression in HDFCs.Since MCP-1 is an important factor to recruit mononuclear cells.IL-10 suppresses the recruitment of these cells to the follicle by down-regulating MCP-1 expression.
Keywords：dental follicle cellsinterleukin-10monocyte chemotactic protein-1tooth eruption
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 613-615,623 )