Cloning and sequencing of the terminal C encoding gene of mouse Periostin
Abstract：AIM: To get the terminal C encoding gene of Periostin from mouse periodontium. METHODS: Total RNA was extracted from the periodontium of adult mouse by acid guanidinium thiocyanata-phenol-chloroform method. The desired DNA product was obtained from the total RNA by RT-PCR with the primers including Oligo(dt) and two gene specific primers. The segment (about 940bp) was inserted into pRSET-B vector and the insered plasmid was transformed into E.coli DH5α.The positive clone was analyzed by restriction endonuclease mapping and DNA sequencing. RESULTS: The restriction endonuclease map and sequence of mouse Periostin functional fragment were consistent with those of the published. CONCLUSION: The terminal C encoding gene of mouse Periostin has been cloned.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 183-185 )