Surface modification of calcium phosphate bioceramics by fibronectin does have positive effects on osteoblasts anchorage
CHEN Xi-zhe
YANG Lian-jia
YANG Wei-dong
Abstract：AIM: To investigate the effects of fibronectin surface modification on porous calcium phosphate bioceramics to osteoblasts adhesion METHOD: Four cuboid porous ceramics（5mm×5mm×5mm）were divided into experimental and control groups randomly. The experimental group was soaked in 100μg/ml recombinant human fibronectin under negative pressure over night. Then both groups were cultivated in vitro simultaneously with osteoblasts derived from bone marrow stem cells of rats for ten days. The morphogenetic pattern and number of the anchored cells were observed under scanning electron microscope. RESULT: There was statistically significant difference of cell numbers between the experimental and the control groups (P<0.01). Many secretory collagen fiber bundles and mineralized nods were observed in the experimental group under high magnifier. CONCLUSION: It seems that the fibronectin surface coating is beneficial to osteoblast anchorage and its osteogenic phenotype.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 163-165 )