Immunohistochemical localization of Smad5 in human dental papillae cell
Abstract：AIM: To investigate the expression and role of Smad5 protein in mediating transforming growth factor-β superfamily(TGF-β) signaling transduction in human dental papillae cells (HDPC). METHODS: Smad5 was stained with immunohistochemical method in HDPC derived from primary culture by treatment with or without BMP-2 or TGF-β1. Difference of the amount of Smad5 was determined by Medical Image Analysis System. RESULTS: The protein of Smad5 was expressed in cytoplasm of HDPC in all the three groups, and also localized in the nucleus of BMP-2 group. Significantly high level of Smad5 was found in BMP-2 group(P<0.01), but low in the control and TGF-β1 group. No significant difference was found between the control group and TGF-β1 group(P>0.05).CONCLUSION: The expression and translocation of the protein Smad5 in HDPC were detected. It suggests that the Smad5 may participates in BMP-2 signaling pathway.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 149-151 )