Establishment of three dimensional cultured model of pulp cell employing the method of tissue engineering
Abstract：AIM: Three-dimensional cultured model of pulp cell was established to provide a tool in the research of pulp disease. METHODS: Pulp cells were cultivated in vitro. Cells were Collected and seeded on three-dimensional framework of polyglycolic acid (PGA). Inverted microscope, scanning electronic microscope and transmission microscope were used to observe the cell growth in the scaffold. RESULTS: Cells adhered on the fiber of PGA after 24 hours, then began to proliferate and grow in the framework of three-dimension. Scanning electronic microscopy showed that cells with spindle shape spread on PGA fiber. Transmission microscopy showed that the microstructure of cell was normal. CONCLUSION: Cells seeded on PGA framework was an efficient way to establish three-dimensional cultured model of pulp cells.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 146-148 )