Study of the effects of two different kinds of stress on osteoblast and its cytoskeleton in vitro
Abstract：AIM: To examine the effect of two differen t kinds of stress on osteoblast and its cytoskeleton in vitro. METHODS: Fluid shear st ress and compressive pressu re were applied on the rat osteoblasts of the passage third respectively. Phase cont rast microscope and Coomassie BB staining were used to observe the characterist ics of cellular shape and cytoskeleton at different intervals. RESULT S : Changes of the cellular shape, the rearrangment of cytoskeleton wer e found after 6 hours of being applied by shear stress. Some dead cells were also observed after 12 hours. While under the compressive pressure (>20kPa) for 12 h ours, the distance between cells increased and no obvious change of cytoskeleton were observed. CONCLUSION: Stress may cause the rearrange ment of cytoskeleton and the change of cellular shape. The compressive pressure, in comparing to the sheas stress, the later, as a kind of physiological like s tress, has less effect on the cellular shape and cytoskeleton of osteoblasts.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 98-100 )