Cloning and sequencing of the gene encoding hBSP mature peptide from human dental pulp cells
Abstract：AIM: To clone and sequence the gene encodin g hBSP mature peptide from human dental pulp cells. METHOD: Total RNA was isolated from cultured human dental pulp cells and desired cDNA fragment was obtained by RT-PCR with two gene specific primers. The segment was inserrted into pBluescript KS vector and the result plasmid was ransformed int o DH5α. The positive clone and sequence were performed. RESULTS: The 900bp specific fragment was obtained. The sequence of the gene encodi ng hBSP mature pepide coincides with that of the references published. CONCLUSION: The gene encoding hBSP mature peptide was obtained from human dental pulp cells. This result will help us to further investigate the expression and function of hBSP.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 86-88 )