Purification and regeneration of rhBMP-2
Abstract：AIM: To study methods for purificat ion and regeneration of E.coli generated rhBMP-2. METHODS: TOP10 strain carrying pBV220-rhBMP-2 recombinant construct was fermented and lysised to prepare inclusion bodies which was then further lysised by ultrosonic followe d by Gel chromatography and ion-chromatographyprocessures to generate high puri ty protein product. different regenerative conditions were investigated to obtai n rhBMP-2 product of higher biological activity measured by ALP activity analysi s. RESULTS: The purity of primitive rhBMP-2 product increa sed by 90% after Gel chromatography and the regenerated protein increased its pu rity by 85% after ion-exchange chromatography. CONCLUSIONS: Gel chromatography and ion-exchange Chromatogaphy help obtain rhBMP product of high purity; 3mol/L urea and 0.75～1.25mol/L NaCl can regenerate rhBMP-2 to a higher level of biological activity.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 73-75 )