Effects of nerve growth factor on the proliferation and cell cycles of human dental papilla mesenchyme cells cultured in vitro
Abstract：AIM: The effects of nerve growth factor on the proliferation andcell cycle of human dental papilla mesenchyme cells were detected to evaluate its possible roles in tooth development. METHODS: 3H-TdR incorporation and flow cytometer analysis. RESULTS: The nerve growth factor showed a significant concentration-dependant stimulation of [3H] thymidine uptake, with a maximum increase at 100U/ml, but inhibit the cellular proliferation at 1000U/ml. Nerve growth factor at 100U/ml increased the percenage of cells in S cycle phase and the percentage of S+G2M. CONCLUSION: nerve growth factor stimulated DNA synthesis of human dental papilla mesenchyme cells, and stimulated the transformation of cells from G1 to S cycle phase.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 38-40 )