Cloning of a new gene expressed differently in human periodontal ligament fibroblast in comparison with gingival fibroblasts
Abstract：AIM: To clone a new gene that is differentially expressed in human PDLF in comparison with GF.METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot. Desired genes were sequenced and then analyzed by computer. RESULTS: We cloned a 818bp new gene from a PDLF cDNA subtractive library. CONCLUSION: We cloned a new gene from the subtuctive htnan PDLF cDNA library.
Keywords：PDLFGFPCRsubtractive hybridizationgene cloning
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 343-346 )