Batch cloning and functional characteristics analysis of genes expressed differently in human periodontal ligament fibroblasts in comparison with gingival fibroblasts
Abstract：AIM: To clone and analyze the functional characteristics of genes expressed differenty in cultured primary human PDLF in comparison with GF. METHODS: Subtractive cDNA library of PDLF was constructed with a modified gene cloning technique which is based on PCR and subtractive hybridization. Genes known to GeneBank were analyzed concerning their functional characteristics. RESULTS: 14 genes were cloned and the 10 known genes are responsible for intracellular process of cell differentiation and matrix synthesis and secretion. CONCLUSION: This study suggests the possible potential of PDLF to differentiate and comparatively more active intracellular protein synthesis and secretion.
Keywords：PDLFGFdifferential expr~sionsubtractive librarygene cloning
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 339-342 )